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07-Jan-2020 04:53

After cells were infected 0, 12, 48, and 72 h, CCK-8 reagent was added to each group of wells at (v/v) per 100 μL medium and incubated for 1 h at 37°C.Measurement was carried out in absorbance at 450 nm with a microplate reader according to the manufacturer’s instruction.It was found that Introduction Renal cell carcinoma (RCC), the most common type of adult kidney malignancy, accounts for 80% of all primary renal neoplasms and is composed of 2%–3% of all malignancies.The occurrence of RCC has increased in recent years; worldwide, ~100,000 patients die of disease every year.

Finally, each sample was resuspended to a volume of 500 μL with 1× binding buffer and was analyzed through flow cytometry within 30 min.

This research studied the involvement of β-catenin in renal cell carcinoma (RCC) cell proliferation, apoptosis, migration, and invasion.



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